Lowicryl® HM20 Non-polar, Hydrophobic, -70ºC Embedding Kit

Lowicryls are low viscosity, highly crosslinked acrylate and methacrylate based embedding media that are designed for use at low-temperature for improved preservation of molecular structure and antigens. They are useful alternative to epoxy resins in immunochemistry.

Description

Lowicryls are low viscosity, highly crosslinked acrylate and methacrylate based embedding media that are designed for use at low-temperature for improved preservation of molecular structure and antigens. They are useful alternative to epoxy resins in immunochemistry.

Lowicryl® HM kits are non-polar and are used to produce high contrast images of completely unstained thin sections by Z-contrast. The HM kits are particularly suitable for darkfield observation because of their relatively low density.

Lowicryl HM20 Non-polar Embedding Kit is designed for use at temperature as low as -70oC.

Kit Contains: 3x225gm of Monomer E, 1x130gm of Crosslinker D and 1×4.8gm of Initiator C (not shown)

Reference(s):
Brown E, Mantell J, Carter D, Tilly G, Verkade P. (2009). Studying intracellular transport using high-pressure freezing and Correlative Light Electron Microscopy. Semin Cell Dev Biol. 20(8):910-9.
Newman GR, Hobot JA. (1999). Resins for combined light and electron microscopy: a half century of development. Histochem J. 31(8):495-505.
Anderson WH, Thompson EW, Zwizinski CW. (1991). A rapid method for the preparation of yeast for immunoelectron microscopy using Lowicryl HM-20. J Electron Microsc Tech. 18(2):172-5.
Hazards: Irritant , Lachrymator , Poison (Absorption)
Handling: Glove, chemical goggles & chemical mask or hood
Storage: Protect from light; Store at room temperature