Polyethylenimine, Linear, MW 25,000 (PEI 25000)
£209.00 – £1,090.60 Price ex. VAT
Pack sizes : 100mg , 1g
Linear Polyethylenimine (PEI 25000) is a high-charge cationic polymer that readily binds with highly anionic substrates. In research, PEI readily binds to DNA and other negatively charged biological molecules, making it one of the most efficient vector carriers available.
Delivery on this product is usually within 7 – 10 days.
Description
Linear Polyethylenimine (PEI 25000) is a high-charge cationic polymer that readily binds with highly anionic substrates. PEI readily binds to DNA and other negatively charged biological molecules, making it one of the most efficient vector carriers available.
PEI 25000 contains some partially acylated amines (N-propionylated) from isomerization-polymerization of 2-ethyl-2-oxazoline followed by acid-hydrolysis of resulting N-propionyl-PEI. For fully deacylated PEI, see Polyethylenimine HCl MAX, Linear, Mw 40,000.
CAS#: 9002-98-6, 26913-06-4
Formula: (CH2CH2NH)n
Molecular Weight: 25,000
Melting Point: 73-75º
Soluble In: hot water, cold water at low pH, methanol, and ethanol.
Insoluble In: benzene, ethyl ether and acetone
Appearance: White to yellow solid
Reference(s):
Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020
Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002
Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9
Hazards: Unknown
Handling: Gloves & fume hood
Storage: Store at room temperature
MSDS / Technical Data Sheets / Product Literature
Additional information
| Size | 1 g, 100 mg |
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