Polyethylenimine HCl MAX, Linear, Mw 40,000 (PEI MAX 40000)
£1,123.85 Price ex. VAT
Quantity: 1g
Linear polyethylenimine HCl MAX, Mw 40,000 (PEI MAX 40000) is a fully hydrolyzed (deacylated), highly water soluble hydrochloride salt of PEI 25000. PEI MAX 40000 contains a longer contiguous ethyleneimine segments resulting in increase in protonable nitrogens than PEI 25000
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Description
Linear polyethylenimine HCl MAX, Mw 40,000 (PEI MAX 40000) is a fully hydrolyzed (deacylated), highly water soluble hydrochloride salt of PEI 25000. PEI MAX 40000 contains a longer contiguous ethyleneimine segments resulting in increase in protonable nitrogens than PEI 25000
PEI MAX 40000 is supplied as a hydrochloride salt for ease of handling and high water solubility. PEI MAX 40000 can be converted into the linear free amine (PEI ~22000) form by neutralization with base.
CAS#: 26338-45-4, 9002-98-6, 26913-06-4
Molecular Weight: 40,000 (~22,000 free base)
Soluble In: Cold and room temperature water
Insoluble in: Common organic solvents (ethanol, acetone, tetrahydrofuran)
Appearance:White to off-white free flowing solid
Reference(s):
Kobayashi, S., Yoshii, K., Hirano, M., Muto, M. & Kariwa, H. A novel reverse genetics system for production of infectious West Nile virus using homologous recombination in mammalian cells. Journal of Virological Methods 240, 14–20 (2017). doi:10.1016/j.jviromet.2016.11.006
Longo, P. a, Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.
Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002
Mann JF, McKay PF, Arokiasamy S, Patel RK, Klein K, Shattock RJ. (2013). Pulmonary delivery of DNA vaccine constructs using deacylated PEI elicits immune responses and protects against viral challenge infection. J Control Release. 170(3):452-9.
Kami, D. et al. Application of magnetic nanoparticles to gene delivery. International Journal of Molecular Sciences 12, 3705–3722 (2011). doi:10.3390/ijms12063705
Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003
Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.
Hazards: Irritant
Handling: Glove, chemical goggles & fume hood
Storage: Store at room temperature
