Picrosirius Red Stain Kit

Picrosirius Red Stain binds specifically to collagen fibrils of varying diameter that is used to distinguish collagen Type I from Type III. Picrosirius Red Stain will quantify the amount of collagen in a given area of myocardial tissue. Picrosirius Red staining is also useful in the quantitative aspect of liver fibrosis by measuring a change in collagen proportion stained as red (Jang et.al., 2014).

500ml kit = stains upto 200 slides

SKU: 24901-500 Categories: , ,

Description

Picrosirius Red Stain binds specifically to collagen fibrils of varying diameter that is used to distinguish collagen Type I from Type III. Picrosirius Red Stain will quantify the amount of collagen in a given area of myocardial tissue. Picrosirius Red staining is also useful in the quantitative aspect of liver fibrosis by measuring a change in collagen proportion stained as red (Jang et.al., 2014).

Kit contains:
Solution A – Phosphomolybdic Acid (250ml or 500ml)
Solution B – Picrosirius Red F3BA Stain (250ml or 500ml)
Solution C – 0.1 N Hydrochloride Acid (250ml or 500ml)

500 ml kit stains up to 200 slides

Reference(s):
Fujita, K. et al. A new rabbit model of impaired wound healing in an X-ray-irradiated field. PLOS ONE 12, e0184534 (2017). doi:10.1371/journal.pone.0184534
Muto, T. et al. Temporary inductions of matrix metalloprotease-3 (MMP-3) expression and cell apoptosis are associated with tendon degeneration or rupture after corticosteroid injectionJournal of Orthopaedic Research 32, 1297–1304 (2014). doi:10.1002/jor.22681
Jang, Y. O. et al. Histological improvement following administration of autologous bone marrow-derived mesenchymal stem cells for alcoholic cirrhosis: a pilot study. Liver International 34, 33–41 (2014). doi:10.1111/liv.12218
Foster, B. L., Sheen, C. R., Hatch, N. E., Liu, J., Cory, E., Narisawa, S., … & Millán, J. L. (2015). Periodontal defects in the A116T knock-in murine model of odontohypophosphatasiaJournal of dental research94(5), 706-714.
Rodrigues, T. L., Nagatomo, K. J., Foster, B. L., Nociti Jr, F. H., & Somerman, M. J. (2011). Modulation of phosphate/pyrophosphate metabolism to regenerate the periodontium: a novel in vivo approachJournal of periodontology82(12), 1757-1766.
Kelly DJ, Chanty A, Gow, RM, Zhang Y, and Gilbert RE (2005) Protein Kinase CInhibition Attenuates Osteopontin Expression, Macrophage Recruitment, and Tubulointerstitial Injury in Advanced Experimental Diabetic NephropathyJ Am Soc Nephrol 16: 1654 –1660.
Wenxuan Liu, Lan Wei-LaPierre, Alanna Klose, Robert T Dirksen, Joe V Chakkalaka Inducible depletion of adult skeletal muscle stem cells impairs the regeneration of neuromuscular junctions eLife  2015;4:e09221
 
Hazards: Irritant
Handling: Gloves, chemical goggles
Storage: Store at room temperature