Polyethylenimine, Linear, MW 25,000 (PEI 25000)

£198.55£1,038.35 Price ex. VAT

Pack sizes : 100mg ,  1g

Linear Polyethylenimine (PEI 25000) is a high-charge cationic polymer that readily binds with highly anionic substrates. In research, PEI readily binds to DNA and other negatively charged biological molecules, making it one of the most efficient vector carriers available.

Delivery on this product is usually within 7 – 10 days.

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Description

Linear Polyethylenimine (PEI 25000) is a high-charge cationic polymer that readily binds with highly anionic substrates. PEI readily binds to DNA and other negatively charged biological molecules, making it one of the most efficient vector carriers available.

PEI 25000 contains some partially acylated amines (N-propionylated) from isomerization-polymerization of 2-ethyl-2-oxazoline followed by acid-hydrolysis of resulting N-propionyl-PEI. For fully deacylated PEI, see Polyethylenimine HCl MAX, Linear, Mw 40,000.

 

CAS#: 9002-98-6, 26913-06-4

Formula: (CH2CH2NH)n

Molecular Weight: 25,000

Melting Point: 73-75º

Soluble In: hot water, cold water at low pH, methanol, and ethanol.
Insoluble In: benzene, ethyl ether and acetone

Appearance: White to yellow solid

 

Reference(s):

Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregationActa Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A. 102(16):5679-84.

Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cellsNucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

 

Hazards: Unknown

Handling: Gloves & fume hood

Storage: Store at room temperature

 

MSDS / Technical Data Sheets / Product Literature

Additional information

Size

1 g, 100 mg