DOTMA Chloride

Synonyms: N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecen-1-yloxy]-1-propanaminium, chloride

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Synonyms: N,N,N-trimethyl-2,3-bis[(9Z)-9-octadecen-1-yloxy]-1-propanaminium, chloride

Key Features:

–        Non-cytotoxic at working concentrations

–        High efficiency and reproducibility

–        Cost effective and easy to use

DOTMA Chloride (N-[1-(2,3-Dioleyloxy)propyl]-N,N,N-trimethylammonium (chloride)) is a proven reagent for the highly efficient liposomal transfection of DNA, RNA, oligonucleotides, and other negatively charged large biomolecules. Several other techniques of introducing genetic material into cells may suffer from inadequate reproducibility, cytotoxicity, inconvenience of process, and low transfection efficiency. DOTMA Cl avoids these drawbacks and works by encapsulating the negatively charged DNA within a spontaneously formed liposomal vesicle. This DOTMA/DNA complex can then fuse with the cell membrane and insert DNA directly into the cytoplasm. This mechanism of action ensures transfection occurs efficiently and with little to no cytotoxicity.

CAS#: 104162-48-3
Formula: C42H84NO2Cl
Molecular Weight: 670.6
Soluble In: Ethanol, chloroform, DMSO, DMF. Insoluble in water.
Appearance: White solid
Ren, T., Song, Y., Zhang, G. et al. Structural basis of DOTMA for its high intravenous transfection activity in mouse. Gene Ther 7, 764–768 (2000).
C F Bennett, M Y Chiang, H Chan, J E Shoemaker and C K Mirabelli. Cationic lipids enhance cellular uptake and activity of phosphorothioate antisense oligonucleotides. Molecular Pharmacology June 1992, 41 (6) 1023-1033;
Felgner, P. L., Gadek, T. R., Holm, M., Roman, R., Chan, H. W., Wenz, M., … Danielsen, M. (1987). Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proceedings of the National Academy of Sciences, 84(21), 7413–7417. doi:10.1073/pnas.84.21.7413
Storage: -20°C (Powder), 4°C (Formulated Solution)