Transporter™ 5 Transfection Reagent

£304.00 – £1,223.60 Price ex. VAT

Transporter™ 5 Transfection Reagent is the ready-to-use form of a Polysciences proprietary linear polyethylenimine (PEI) derivative.  This ready-to-use transfection reagent condenses DNA into positively charged complexes which readily enter the cell by endocytosis. The Transporter™ 5-DNA complex is highly resistant to endosomal degradation, allowing high transfection efficiency.

Delivery on this product is usually within 7 – 10 days.

 

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Description

 Transporter™ 5 Transfection Reagent is the ready-to-use form of a Polysciences proprietary linear polyethylenimine (PEI) derivative.  This ready-to-use transfection reagent condenses DNA into positively charged complexes which readily enter the cell by endocytosis. The Transporter™ 5-DNA complex is highly resistant to endosomal degradation, allowing high transfection efficiency.

Transporter™ 5 transfection reagent effectively introduces DNA into HEK-293, CHO, COS, HeLa, insect (Sf9 and Sf21) and other eukaryotic cell lines. Transporter™ 5 is most compatible with plasmids up to 135,000 nucleotides in size, although larger plasmids can be used with some success.

Appearance:  Liquid

 

Reference(s):

Rajendra, Y., Kiseljak, D., Baldi, L., Wurm, F. M. and Hacker, D. L. (2015), Transcriptional and post-transcriptional limitations of high-yielding, PEI-mediated transient transfection with CHO and HEK-293E cells. Biotechnol Progress, 31: 541–549. doi: 10.1002/btpr.2064

Longo, P. A., Kavran, J. M., Kim, M. & Leahy, D. J. Transient Mammalian Cell Transfection wtih Polyethylenimine (PEI). Methods Enzymology 529, 227–240 (2013). doi:10.1016/B978-0-12-418687-3.00018-5.

Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

Backliwal, G., Hildinger, M., Hasija, V. and Wurm, F. M. (2008), High-density transfection with HEK-293 cells allows doubling of transient titers and removes need for a priori DNA complex formation with PEI. Biotechnol. Bioeng., 99: 721–727. doi: 10.1002/bit.21596

Backliwal, G. et al. Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions. Nucleic Acids Research 36, e96–e96 (2008). doi:10.1093/nar/gkn423

Tom, R., Bisson, L. & Durocher, Y. Transfection of HEK293-EBNA1 cells in suspension with linear PEI for production of recombinant proteins. Cold Spring Harbor Protocols 3, 1–5 (2008). doi:10.1101/pdb.prot4977

Choosakoonkriang, S., Lobo, B. A., Koe, G. S., Koe, J. G. and Middaugh, C. R. (2003), Biophysical characterization of PEI/DNA complexes. J. Pharm. Sci., 92: 1710–1722. doi: 10.1002/jps.10437

Expired Patent: US6013240; Nucleic Acid Containing Composition, Preparation, and Uses of the Same

 

Hazards:  Harmless, use normal precautions

Handling: Gloves & chemical googles

Storage: Store at 4 degree C; do not freeze. Shipped at room temperature (RT).

 

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